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Details on  PrimeTime   qPCR  assay primers

Journal: Nature Communications

Article Title: ADAM9 promotes type I interferon-mediated innate immunity during encephalomyocarditis virus infection

doi: 10.1038/s41467-024-48524-6

Figure Lengend Snippet: Details on PrimeTime qPCR assay primers

Article Snippet: Equal RNA amounts of total RNA were used for qRT-PCR analysis using the SuperScript III Platinum One-Step qRT-PCR kit with ROX and predesigned PrimeTime qPCR Probe Assays (IDT, see Table ) on a QuantStudio 6 (Applied Biosystems). mRNA expression was normalized to the levels of GAPDH and expressed relative to the values for control cells using the ΔΔCt method.

Techniques: